one-way anova with dunnett’s post hoc multiple comparisons tests (graphpad prism 6 software)  (GraphPad Software Inc)

Journal: Journal of Extracellular Biology

Article Title: Growth phase matters: Boosting immunity via Lacticasebacillus‐derived membrane vesicles and their interactions with TLR2 pathways

doi: 10.1002/jex2.169

Figure Lengend Snippet: Pro‐inflammatory (NO, TNFα, IL‐6) and immunoregulatory (IL‐10) mediators (a–d, respectively) produced by RAW 264.7 treated with Lacticaseibacillus rhamnosus CCM7091 membrane vesicles (MVs) in three concentrations (10 7 , 10 5 and 10 3 MVs/cell). MVs were isolated at different times of cultivation: 6, 12 and 48 h (MV6, MV12, MV48); PBS was used as negative control (Ctrl), and Escherichia coli lipopolysaccharide (LPS) was used as positive control. The box plots show medians (lines), inter‐quartile ranges (boxes) and minimum and maximum values (whiskers); ANOVA was followed by Dunnett's multiple comparisons test versus negative control; statistical significance was assessed at * p < 0.05; n = 3.

Article Snippet: For statistical analysis, ANOVA followed by Dunnett's multiple comparison test in GraphPad Prism 6 (GraphPad Software, San Diego, California, USA) was used; three independent experiments were performed.

Techniques: Produced, Membrane, Isolation, Negative Control, Positive Control

Journal: Journal of Extracellular Biology

Article Title: Growth phase matters: Boosting immunity via Lacticasebacillus‐derived membrane vesicles and their interactions with TLR2 pathways

doi: 10.1002/jex2.169

Figure Lengend Snippet: (a) Relative optical density and representative Western blot of lipoteichoic acid (LTA) detected in Lacticaseibacillus rhamnosus CCM7091 membrane vesicles (MVs) isolated after 6, 12, 48 h (MV6, MV12, MV48). PBS was used as a negative control. Bars represent means ± SD; statistical significance was assessed at * p < 0.05; ordinary one‐way ANOVA was followed by Dunnett's multiple comparison test versus negative control; n = 3. (b), (c) Human cell receptors involved in the recognition of Lacticaseibacillus rhamnosus CCM7091 membrane vesicles (MVs). Effects of MVs isolated after 6, 12, 48 h (MV6, MV12, MV48) on activation of TLR2/CD14 (a) and TLR6/2 (b) expressed on human embryonic kidney cells HEK‐293 (concentration 10 5 MVs/cell). The recognition abilities are compared to the negative control (PBS), and appropriate positive controls are depicted (HKLM for HEK293 TLR2/CD14, FSL‐1 for HEK293 TLR6/2). Results were evaluated based on IL‐8 production activity in cell media. The box plots show medians (lines), inter‐quartile ranges (boxes) and minimum and maximum values (whiskers); data normalization was performed using Z ‐score; statistical analysis was performed using the ANOVA and the significance was assessed at * p < 0.05; n = 4.

Article Snippet: For statistical analysis, ANOVA followed by Dunnett's multiple comparison test in GraphPad Prism 6 (GraphPad Software, San Diego, California, USA) was used; three independent experiments were performed.

Techniques: Western Blot, Membrane, Isolation, Negative Control, Comparison, Activation Assay, Concentration Assay, Activity Assay